Fig. 3

Identification of the potential target genes of SUV39H2. a The pathway enrichment assay was conducted according to the KEGG pathway database, and the top 20 pathways in the assay are enlisted in the figure. b mRNA expression of the indicated genes in control or SUV39H2 knockdown A549 cells. The error bars represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 (two-tailed unpaired t test). Each sample with the indicated primers was used for the PCR analyses in three replicates, and the experiment was performed in triplicate. c The protein level of STOM and TPM4 and H3K9me3 level in SUV39H2 knockdown A549 cells. d The protein level of STOM and TPM4 in surgically removed lung adenocarcinoma tissue samples and their paired normal adjacent tissue samples. e, f The correlation analysis of SUV39H2 with OPTN or STOM with regard to mRNA expression. g The OPTN promoter-mediated luciferase activity in indicated cells. Each group was composed of six samples and each experiment was repeated three times. h The ChIP-qPCR assay conducted with indicated antibodies in each group of cells. **p < 0.01. i Kaplan-Meier plots of overall survival associated with mRNA expression levels of SUV39H2 and OPTN in TCGA lung adenocarcinomas