Fig. 3

Confirmation of changes in DNA methylation in colon tumors using TCGA cohort. a Schematic of examining DNA methylation changes between seven colon tumors vs. seven matched normal tissues as assayed by HM450 methylation arrays. b Distribution of differentially methylated CpG sites between matched normal colon vs. colon tumors examined by HM450 methylation arrays. c Distribution of differentially methylated CpG sites in colon tumors across the human genome. Using a stringent cutoff of > 30% change in DNA methylation, we identified ~ 117,451 CpG sites that are differentially methylated in colon tumors vs. normal colon as assayed by HM450 arrays. We further separated CpG sites that become hyper- vs. hypomethylated, and their genomic location (gene promoter, gene body, or intergenic region)