Table 1 Primers designed for multiplex PCR
Gene | Fragment | Forward primer | Reverse primer |
|---|---|---|---|
CYP24A1 | CYP24A1 _1 | TAGAGGAGGGYGGAGTGGTTT | CACACCCRATAAACTCCRAACTTC |
CYP24A1 _2 | GGAGATAATTTTTAGGAAGTTATGYGAAGTT | CACTTCAATCCAAACTAAAAATATCTAACTC | |
CYP27A1 | CYP27A1 _1 | TTGGTTTYGTGGGGGTAGAG | CACCRCRTCCCTCTCCTACAA |
CYP27A1 _2 | GGAGGGTYGAGTAAAGGTTAGTTAGAT | AAAACCTATCCCRATATAAAACTTCC | |
CYP27A1 _3 | ATTTTGGGYGGGGGTGTAG | CCCTCCAAAAATCAAATAACTAACC | |
CYP27A1 _4 | TTTTYGGATTGATTTYGGAGTTAGT | ACTATACRTTTTCCRTACTATATTACTCTTTCC | |
CYP27A1 _5 | GGTTGAGATTAGATTTYGTAGATGATG | ACCAACTATACCATCCTACTAAATCCT | |
CYP27B1 | CYP27B1 _1 | GGTTGAGATATGATGTTTAGGAGAAG | TCCCTTCCTACCTACAACTCRTATA |
CYP27B1 _2 | TTTGGYGTGGGTATAGGTTAAGTTG | CTCACRCAATAAACAATCCRCAAAC | |
CYP27B1 _3 | GAGTTGTTGYGATAGGAGGGATT | CAACCRACCTCCCACCA | |
CYP2R1 | CYP2R1 _1 | TTAATGGGAGTATGGTAGGGTTG | AAAAACCCATCRACCRCCTCTA |
CYP2R1 _2 | GGTAGGGAGGGTYGTTAGGTTG | CAAAACTAAATCRCCTCRAAACCTC | |
CYP2R1 _3 | TGTAGGGGGAGTTTYGTTTTTGT | CAAACACCRAAAAACCTACTATTAACC | |
CYP2R1 _4 | GGAAAATTAAGGYGTTTTGAGTTTTA | CACACAAAAAACRCCTTTTAAATATCTAC | |
VDR | VDR _1 | GTAGTTATTTATAATTTTAGGTTTTAGGAGGTAG | CTCAACCTAATCCCACAAATTAAAA |
VDR _2 | AGGTGATATYGGGTGGGAGTAAT | CCACCTAAACTAACCAAACCAA | |
VDR _3 | GGTGTTAGTYGGTAGGYGTTTTTTAG | CATAAAACAAAACACRCTTCTACCCT | |
VDR _4 | TTTYGATTAATATAGGTTGAAGYGGGTA | CCCACAAATCCAATCCTCTC | |
VDR _6 | GAATTYGGGAGTAGYGGGAAAG | TACTAAACACTATATTAACRAAACATTTCTCC |