TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells

Fan, Jiangxia; Zhang, Yan; Mu, Junhao; He, Xiaoqian; Shao, Bianfei; Zhou, Dishu; Peng, Weiyan; Tang, Jun; Jiang, Yu; Ren, Guosheng; Xiang, Tingxiu

doi:10.1186/s13148-018-0535-7

Table 1 List of primers used in this study

From: TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells

PCR	Primer	Sequence (5′-3′)	Product size (bp)	PCR Cycles	Annealing temperature (°C)
RT-PCR	TET1F	AGGACCAAGTGTTGCTGCTGT	219	32	55
	TET1R	ATCACAGCAGTTGGACAGTGG	219	32	55
	β-actinF	TCCTGTGGCATCCACGAAACT	315	23	55
	β-actinR	GAAGCATTTGCGGTGGACGAT	315	23	55
qRT-PCR	DKK1F	CTGCATGCGTCACGCTATGT	161		60
	DKK1R	AGGTGGTTCTTCTGGAATAC	161		60
	DKK2F	ACCCGCTGCAATAATGGAATC	99		60
	DKK2R	ATGGTTGCGATCTCTATGCCG	99		60
	Snail1F	GAGGCGGTGGCAGACTAG	159		60
	Snail1R	GACACATCGGTCAGACCAG	159		60
	DKK3F	CACCCTCAATGAGATGTTCC	161		60
	DKK3R	TGGTCTCATTGTGATAGCTG	161		60
	DACT1F	CTGGAGGAGAAGTTCTTGGA	161		60
	DACT1R	TCCAGGTGCTCTTCAGATGT	161		60
	DACT2F	AGCCGTGGGGCACATTCTG	173		60
	DACT2R	CCAGGTCCTGCCGATACTTG	173		60
	SFRP1F	ACGAGTTGAAATCTGAGGCCATC	197		60
	SFRP1R	ACAGTCAGCCCCATTCTTCAG	197		60
	SFRP2F	ATCCTGGAGACCAAGAGCAAGAC	142		60
	SFRP2R	TGACCAGATAGGGCGCGTTGATG	142		60
	WNT5BF	AAATGCCACGGCGTCTCG	163		60
	WNT5BR	GGGTGAAGCGGCTGTTGA	163		60
	WNT3F	ACGAGAACTCCCCCAACTTT	170		60
	WNT3R	GATGCAGTGGCATTTTTCCT	170		60
	WNT5AF1	CGGTGTACAACCTGGCTGATG	101		60
	WNT5AR1	CACCTTGCGGAAGTCTGCC	101		60
	WNT7AF	CTGGAACTGCTCTGCACTGGGA	129		60
	WNT7AR	GTACAGGCAGCTGTGATGGCGT	129		60
	WNT7BF	TTTGGCGTCCTCTACGTGAAG	145		60
	WNT7BR	CCCCGATCACAATGATGGCA	145		60
	EcadF	TACACTGCCCAGGAGCCAGA	103		60
	EcadR	TGGCACCAGTGTCCGGATTA	103		60
	NcadF	CGAATGGATGAAAGACCCATCC	174		60
	NcadR	GGAGCCACTGCCTTCATAGTCAA	174		60
	β-actinF1	GTCTTCCCCTCCATCGTG	113		60
	β-actinR1	AGGGTGAGGATGCCTCTCTT	113		60
MSP	TET1m4	GTCGGTAGGCGTTTTTCGC	173	40	60
	TET1m8	CCCAACTCACCGCTAACCG	173	40	60
	TET1u4	GAGTTGGTAGGTGTTTTTTGT	175	40	58
	TET1u8	CCCAACTCACCACTAACCA	175	40	58
	DKK1m1	ATTTTGTAGTCGAATCGGTAC	127	40	60
	DKK1m2	CCGAATAACTCCCGCTACG	127	40	60
	DKK1u1	TGATTTTGTAGTTGAATTGGTAT	131	40	58
	DKK1u2	ACCCAAATAACTCCCACTACA	131	40	58
	DKK2m1	AGAGTTAAATCGTCGAGATTTC	146	40	60
	DKK2m2	CTAAAAACAATCAAATACGAAACG	146	40	60
	DKK2u1	GGAGAGTTAAATTGTTGAGATTTT	149	40	58
	DKK2u2	ACTAAAAACAATCAAATACAAAACA	149	40	58
	DKK3m3	TTTCGGGTATCGGCGTTGTC	148	40	60
	DKK3m4	ACTAAACCGAATTACGCTACG	148	40	60
	DKK3u3	GTTTTTTTGGGTATTGGTGTTGTT	135	40	58
	DKK3u4	CAACTAAACCAAATTACACTACA	135	40	58
	DACT1m3	CGGGATAGTAGTAGTCGGC	118	40	60
	DACT1m4	CGCTAAAACTACGACCGCG	118	40	60
	DACT1u3	GTTGGGATAGTAGTAGTTGGT	123	40	58
	DACT1u4	AAACACTAAAACTACAACCACA	123	40	58
	DACT2m3	CGTGTAGATTTCGTTTTTCGC	200	40	60
	DACT2m4	CCGAAAATCCGCCCGACG	200	40	60
	DACT2u3	TGTGTGTAGATTTTGTTTTTTGT	203	40	58
	DACT2u4	CCCCAAAAATCCACCCAACA	203	40	58
	SFRP1m1	TGTAGTTTTCGGAGTTAGTGTCGCGC	126	40	60
	SFRP1m2	CCTACGATCGAAAACGACGCGAACG	126	40	60
	SFRP1u1	GTTTTGTAGTTTTTGGAGTTAGTGTTGTGT	137	40	58
	SFRP1u2	CTCAACCTACAATCAAAAACAACACAAACA	137	40	58
	SFRP2m1	GGAGTTTTTCGGAGTTGCGC	128	40	60
	SFRP2m2	CTCTTCGCTAAATACGACTCG	128	40	60
	SFRP2u1	GTTGGAGTTTTTTGGAGTTGTGT	133	40	58
	SFRP2u2	CTCTCTTCACTAAATACAACTCA	133	40	58
BGS	TET1BGS1	TTGTTTTTTTATTGTGGATTTTTG	384	40	60
BGS	TET1BGS2	AACCCACCCCTAAAACAAC	384	40	60
Chip-PCR	DACT2F	CGTGCAGACCCCGCCCTC	113		60
	DACT2R	GATCCCGAGCTGTGTCGCG	113		60
	SFRP2F	TGTCCCGCTTCTCCGCG	98		60
	SFRP2R	GAGTTCGAGCTTGTCCCG	98		60
	Wnt5AF	CTCTCCGTGGAACAGTTGC	136		60
	Wnt5AR	GCAGAGCTGGGATGCGC	136		60

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ISSN: 1868-7083

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