Fig. 1
From: Effects of novel HDAC inhibitors on urothelial carcinoma cells
Effects of HDACi 19e, 19h, and 19i on urothelial carcinoma and control cell lines. HDACi were applied to UC cell lines VM-CUB1, UM-UC-3, and 639-V as well as control cell lines HEK293 (non-urothelial) and HBLAK (urothelial). a Dose-response curves after 24, 48, and 72 h of treatment of UCCs with 0.5, 2, und 5 μM of each HDACi. The calculated significances refer to the DMSO solvent control (*p < 0.05). Data shown are mean from n = 3. b Dose-response curve of UCCs VM-CUB1, UM-UC-3, 639-V, HEK293, and HBLAK after 72 h of treatment with 0.5–5 μM 19i. Data shown are mean from n = 4. c Clonogenicity following 19i treatment of VM-CUB1, UM-UC-3, 639-V, HEK293, and HBLAK. Cells were treated with DMSO, 2.5 μM SAHA, or 2 μM 19i for 48 h, replated at clonal density, cultured for 2 weeks, and stained with Giemsa. d Changes in cell cycle distribution after 24 or 48 h of treatment with 19i. Cell cycle changes and amount of apoptotic cells (as sub-G1 fraction) determined by flow cytometry following 2 μM 19i or 2.5 μM SAHA treatment in VM-CUB1 UM-UC-3, 639-V, HEK293, and HBLAK. DMSO is the solvent control. Data shown are representative of triplicates