Fig. 1
From: Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
Phenotypic and epigenetic characterization of engrafted human hematopoietic cells. a Flow cytometric analysis of bone marrow (BM) 19 weeks after transplantation of human CD34+ cells into NSGW41 mice. Erythroid cells (Ter119+ or CD235+) were excluded, and human CD45+ (hCD45+) cells were analyzed for the expression of cell type-specific surface markers of B cells (CD19), T cells (CD3), monocytes (CD14), NK cells (CD56), and granulocytes (CD16). b Cellular composition of hCD45+ cells in BM of five humanized mice. Cells described as “others” include stem and progenitor cells, myeloid progenitors, and dendritic cells. c Unsupervised hierarchical clustering of global DNA methylation (DNAm) profiles of various hematopoietic cell types purified from peripheral blood (monocytes, granulocytes, and lymphocytes; GSE35069) or umbilical cord blood (CB; GSE40799) compared to those of hCD45 sorter purified cells from BM of humanized mice (HuMice; GSE103010). d Principal component analysis (PCA) of the same hematopoietic subsets described in c. PBMCs, peripheral blood mononuclear cells