Epigenome-wide association study of metabolic syndrome in African-American adults

Clinical Epigenetics

Table 2 Top CpG methylation sites associated with metabolic syndrome status in HyperGEN discovery and REGARDS replication and meta-analysis in basic and adjusted models

Study	Model	CpG	Chr	Gene	Location	β (SE)	p value
Discovery set
HyperGEN (N = 614 (basic) and 606 (fully adjusted))	Basic	cg06500161	21	ABCG1	43656587	0.017 (2.05 × 10^− 3)	1.08 × 10⁻⁸
	Basic	cg06638433	17	IGF2BP1	47075175	0.01 (1.90 × 10⁻³)	2.05 × 10⁻⁸
	Fully adjusted	cg06500161	21	ABCG1	43656587	0.018 (3.20 × 10⁻³)	2.60 × 10⁻⁸
	Fully adjusted	cg06638433	17	IGF2BP1	47075175	0.01 (2.01 × 10⁻³)	3.10 × 10⁻⁷
Replication set
REGARDS (N = 69)	Basic	cg06500161	21	ABCG1	43656587	0.029 (8.94 × 10⁻³)	0.001
		cg06638433	17	IGF2BP1	47075175	− 0.024 (0.012)	0.05
	Fully adjusted	cg06500161	21	ABCG1	43656587	0.032 (9.0 × 10⁻³)	4.7 × 10⁻⁴
		cg06638433	17	IGF2BP1	47075175	− 0.026 (0.012)	0.035
Meta-analysis
HyperGEN and REGARDS	Basic	cg06500161	21	ABCG1	43656587	N/A	1.54 × 10⁻¹⁰
	Fully adjusted	cg06500161	21	ABCG1	43656587	N/A	2.15 × 10^− 10
	Basic	cg06638433	17	IGF2BP1	47075175	N/A	1.29 × 10⁻⁷
	Full adjusted	cg06638433	17	IGF2BP1	47075175	N/A	1.55 × 10⁻⁶

Basic models refer to linear mixed models with metabolic syndrome as a predictor and CpG β score as the outcome, adjusted for age, sex, center, first four principal components, and five estimated cell proportions. Full adjusted models were additionally adjusted for smoking and alcohol use. Meta-analysis p values are from Han and Eskin [22] random effects models

ISSN: 1868-7083