Fig. 3

a Knockdown of UHRF1 in indicated cell lines. Relative gene expression in cells transfected with siRNA against UHRF1 compared to negative control (siNTC) after 48 h is shown. Data represents average of three independent experiments. b Western blot analysis of HepT1, HepG2, and HUH6 cells 48 h after UHRF1 knockdown. c Relative expression levels of indicated genes in HUH6 cells 48 h after UHRF1 knockdown compared to negative control. The average of three independent experiments is shown. d Methylation status (U, unmethylated; M, methylated) of the HHIP, IGFBP3, and SFRP1 promoter region 48 h after UHRF1 knockdown was determined by MSP in HUH6 cells. Representative images of MSP experiments are shown. e Pyrosequencing results of promoter regions of indicated genes in HUH6 cells 48 h after UHRF1 knockdown of three independent experiments are shown. f ChIP analyses in HUH6 cells 48 h after UHRF1 knockdown. The relative enrichment of H3K9me2 at the indicated gene promoter regions is shown. g Cell viability of HepG2, HepT1, and HUH6 cells as evaluated by MTT assay at the indicated time points after UHRF1 knockdown. Values represent means ± standard deviation of three independent experiments performed in duplicates. Statistical significance of all experiments was calculated using t test (p < 0.05)