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Table 3 Mean observed CpG site methylation in vascular smooth muscle cell DNA after bisulphite sequencing of candidate genes after consideration of sex and smoking status as co-variates of methylation (total n = 24 AAAs, 16 male controls and 16 smoking controls—Additional file 1: Table S2)

From: SMYD2 promoter DNA methylation is associated with abdominal aortic aneurysm (AAA) and SMYD2 expression in vascular smooth muscle cells

Gene, chromosome and RefSeq ID RefSeq ID coordinate Meth % ± SD—all male smokers Smokers only meth % ± SD Smokers only meth difference (% ± SE) Men only meth % ± SD Men only meth difference (% ± SE) Smokers only P value Men only P value
(CpG site) (AAA—n = 24) (Controls—n = 16) n = 16 (Controls—n = 16) n = 16 (24 AAAs vs 16 controls) (24 AAAs vs 16 controls)
ERG 5389 19.00 (3.48) 14.43 (3.99) 4.57 (1.25) 15.07 (4.65) 3.93 (1.32) 0.0011 0.0059
Chr: 21 5393 19.43 (3.72) 14.86 (3.65) 4.58 (1.25) 14.80 (3.82) 4.63 (1.25) 0.0013 0.0009
NG_029732.1 5400 21.26 (5.76) 17.36 (11.73) 3.90 (2.88) 16.87 (11.58) 4.39 (2.82) 0.0011 0.0007
5402 20.43 (5.45) 15.14 (3.55) 5.29 (1.64) 14.80 (3.73) 5.63 (1.61) 0.0009 0.0004
5405 19.96 (4.15) 15.50 (3.54) 4.46 (1.33) 14.93 (3.65) 5.02 (1.32) 0.0014 0.0003
5408 20.13 (4.20) 15.36 (3.69) 4.77 (1.36) 14.87 (11.57) 5.26 (1.33) 0.0007 0.0002
5429 24.13 (5.26) 19.21 (2.83) 4.92 (1.53) 18.73 (3.26) 5.40 (1.52) 0.0028 0.0010
5440 24.65 (4.49) 20.07 (2.92) 4.58 (1.35) 19.60 (3.48) 5.05 (1.37) 0.0010 0.0003
5442 24.57 (5.12) 19.86 (2.51) 4.71 (1.47) 19.40 (3.36) 5.17 (1.50) 0.0009 0.0006
5458 27.22 (4.88) 22.93 (2.49) 4.29 (1.41) 22.40 (3.31) 4.82 (1.44) 0.0030 0.0010
5468 28.35 (6.01) 23.50 (2.34) 4.85 (1.68) 22.80 (3.39) 5.55 (1.71) 0.0127 0.0045
5474 29.39 (5.55) 25.36 (2.56) 4.03 (1.58) 24.53 (3.70) 4.86 (1.63) 0.0125 0.0037
5507 30.83 (5.57) 26.21 (1.85) 4.61 (1.54) 25.20 (3.36) 5.63 (1.60) 0.0040 0.0008
IL6R Chr: 1 3570 93.17 (5.62) 78.31 (22.41) 14.85 (4.76) 75.37 (23.77) 17.79 (5.02) NS 0.0037
NG_012087.1 3676 84.92 (10.89) 75.56 (16.55) 9.35 (4.33) 73.63 (16.97) 11.29 (4.39) NS NS
SERPINB9 2889674 51.00 (16.19) 66.27 (15.98) −15.27 (5.30) 64.07 (16.84) −13.07 (5.41) 0.0037 0.0120
Chr: 6 2889644 20.46 (20.29) 8.13 (9.93) 12.33 (5.63) 9.27 (11.59) 11.19 (5.76) 0.0139 0.0230
NC_000006.12 2889627 71.67 (18.37) 84.93 (12.93) −13.27 (5.44) 82.13 (15.91) −10.47 (5.76) 0.0015 0.0100
2889620 62.88 (19.54) 80.00 (15.17) −17.13 (5.95) 81.00 (14.06) −18.13 (5.84) 0.0018 0.0007
2889612 70.33 (19.08) 80.27 (20.23) −9.933 (6.43) 78.93 (19.58) −8.60 (6.34) 0.0081 0.0168
2889568 76.58 (19.21) 88.33 (12.19) −11.75 (5.56) 87.67 (9.31) −11.08 (5.33) 0.0003 0.0018
2889564 78.67 16.34) 87.80 (11.82) −9.13 (4.88) 90.47 (8.57) −11.80 (4.59) 0.0039 0.0002
SMYD2 214280412 39.50 (14.74) 59.00 (17.12) −19.50 (6.64) 55.38 (17.33) −15.88 (6.37) 0.0002 0.0015
Chr: 1 214280441 32.91 (10.53) 48.57 (17.80) −15.66 (5.37) 41.56 (14.78) −8.64 (4.64) 0.0004 NS
NC_000001.11 214280507 38.78 (12.67) 50.14 (8.61) −11.36 (5.14) 50.13 (7.97) −11.34 (4.81) 0.0012 0.0009
214280600 37.04 (11.02) 47.57 (10.63) −10.53 (4.72) 45.78 (12.02) −8.73 (4.44) 0.0001 0.0020
  1. NS not significant