Table 3 Mean observed CpG site methylation in vascular smooth muscle cell DNA after bisulphite sequencing of candidate genes after consideration of sex and smoking status as co-variates of methylation (total n = 24 AAAs, 16 male controls and 16 smoking controls—Additional file 1: Table S2)
Gene, chromosome and RefSeq ID | RefSeq ID coordinate | Meth % ± SD—all male smokers | Smokers only meth % ± SD | Smokers only meth difference (% ± SE) | Men only meth % ± SD | Men only meth difference (% ± SE) | Smokers only P value | Men only P value |
|---|---|---|---|---|---|---|---|---|
(CpG site) | (AAA—n = 24) | (Controls—n = 16) | n = 16 | (Controls—n = 16) | n = 16 | (24 AAAs vs 16 controls) | (24 AAAs vs 16 controls) | |
ERG | 5389 | 19.00 (3.48) | 14.43 (3.99) | 4.57 (1.25) | 15.07 (4.65) | 3.93 (1.32) | 0.0011 | 0.0059 |
Chr: 21 | 5393 | 19.43 (3.72) | 14.86 (3.65) | 4.58 (1.25) | 14.80 (3.82) | 4.63 (1.25) | 0.0013 | 0.0009 |
NG_029732.1 | 5400 | 21.26 (5.76) | 17.36 (11.73) | 3.90 (2.88) | 16.87 (11.58) | 4.39 (2.82) | 0.0011 | 0.0007 |
5402 | 20.43 (5.45) | 15.14 (3.55) | 5.29 (1.64) | 14.80 (3.73) | 5.63 (1.61) | 0.0009 | 0.0004 | |
5405 | 19.96 (4.15) | 15.50 (3.54) | 4.46 (1.33) | 14.93 (3.65) | 5.02 (1.32) | 0.0014 | 0.0003 | |
5408 | 20.13 (4.20) | 15.36 (3.69) | 4.77 (1.36) | 14.87 (11.57) | 5.26 (1.33) | 0.0007 | 0.0002 | |
5429 | 24.13 (5.26) | 19.21 (2.83) | 4.92 (1.53) | 18.73 (3.26) | 5.40 (1.52) | 0.0028 | 0.0010 | |
5440 | 24.65 (4.49) | 20.07 (2.92) | 4.58 (1.35) | 19.60 (3.48) | 5.05 (1.37) | 0.0010 | 0.0003 | |
5442 | 24.57 (5.12) | 19.86 (2.51) | 4.71 (1.47) | 19.40 (3.36) | 5.17 (1.50) | 0.0009 | 0.0006 | |
5458 | 27.22 (4.88) | 22.93 (2.49) | 4.29 (1.41) | 22.40 (3.31) | 4.82 (1.44) | 0.0030 | 0.0010 | |
5468 | 28.35 (6.01) | 23.50 (2.34) | 4.85 (1.68) | 22.80 (3.39) | 5.55 (1.71) | 0.0127 | 0.0045 | |
5474 | 29.39 (5.55) | 25.36 (2.56) | 4.03 (1.58) | 24.53 (3.70) | 4.86 (1.63) | 0.0125 | 0.0037 | |
5507 | 30.83 (5.57) | 26.21 (1.85) | 4.61 (1.54) | 25.20 (3.36) | 5.63 (1.60) | 0.0040 | 0.0008 | |
IL6R Chr: 1 | 3570 | 93.17 (5.62) | 78.31 (22.41) | 14.85 (4.76) | 75.37 (23.77) | 17.79 (5.02) | NS | 0.0037 |
NG_012087.1 | 3676 | 84.92 (10.89) | 75.56 (16.55) | 9.35 (4.33) | 73.63 (16.97) | 11.29 (4.39) | NS | NS |
SERPINB9 | 2889674 | 51.00 (16.19) | 66.27 (15.98) | −15.27 (5.30) | 64.07 (16.84) | −13.07 (5.41) | 0.0037 | 0.0120 |
Chr: 6 | 2889644 | 20.46 (20.29) | 8.13 (9.93) | 12.33 (5.63) | 9.27 (11.59) | 11.19 (5.76) | 0.0139 | 0.0230 |
NC_000006.12 | 2889627 | 71.67 (18.37) | 84.93 (12.93) | −13.27 (5.44) | 82.13 (15.91) | −10.47 (5.76) | 0.0015 | 0.0100 |
2889620 | 62.88 (19.54) | 80.00 (15.17) | −17.13 (5.95) | 81.00 (14.06) | −18.13 (5.84) | 0.0018 | 0.0007 | |
2889612 | 70.33 (19.08) | 80.27 (20.23) | −9.933 (6.43) | 78.93 (19.58) | −8.60 (6.34) | 0.0081 | 0.0168 | |
2889568 | 76.58 (19.21) | 88.33 (12.19) | −11.75 (5.56) | 87.67 (9.31) | −11.08 (5.33) | 0.0003 | 0.0018 | |
2889564 | 78.67 16.34) | 87.80 (11.82) | −9.13 (4.88) | 90.47 (8.57) | −11.80 (4.59) | 0.0039 | 0.0002 | |
SMYD2 | 214280412 | 39.50 (14.74) | 59.00 (17.12) | −19.50 (6.64) | 55.38 (17.33) | −15.88 (6.37) | 0.0002 | 0.0015 |
Chr: 1 | 214280441 | 32.91 (10.53) | 48.57 (17.80) | −15.66 (5.37) | 41.56 (14.78) | −8.64 (4.64) | 0.0004 | NS |
NC_000001.11 | 214280507 | 38.78 (12.67) | 50.14 (8.61) | −11.36 (5.14) | 50.13 (7.97) | −11.34 (4.81) | 0.0012 | 0.0009 |
214280600 | 37.04 (11.02) | 47.57 (10.63) | −10.53 (4.72) | 45.78 (12.02) | −8.73 (4.44) | 0.0001 | 0.0020 |