Table 5 The fluorescence peak height of a dilution of methylated template in the background of unmethylated template and their correlation coefficiency of different gene standards for methylation analysis of HRM-PCR
Genes | Standards | ||||||
|---|---|---|---|---|---|---|---|
Fluorescence peak height | Correlation coefficient | ||||||
0% | 1% | 25% | 50% | 75% | 100% | ||
ESBSs | 69.20 | 51.00 | 42.50 | 33.60 | 21.90 | 5.80 | 0.963 |
L1-1 | 76.10 | 42.20 | 35.70 | 24.50 | 14.80 | 5.10 | 0.971 |
L1-2 | 66.20 | 46.70 | 38.20 | 24.00 | 12.30 | 5.80 | 0.983 |
L1-3 | 56.30 | 48.80 | 40.20 | 28.50 | 12.30 | 3.60 | 0.976 |
L1-4 | 77.80 | 47.60 | 32.80 | 25.40 | 47.20 | 7.20 | 0.919 |
L1-5 | 89.40 | 70.90 | 55.00 | 39.00 | 15.00 | 6.90 | 0.953 |
L1-6 | 66.90 | 49.30 | 32.10 | 25.00 | 12.30 | 2.50 | 0.989 |
L1-7 | 60.40 | 55.00 | 41.60 | 18.00 | 12.00 | 2.70 | 0.963 |
L2-1 | 70.30 | 51.90 | 45.00 | 32.50 | 19.30 | 6.10 | 0.967 |
L2-2 | 69.80 | 44.80 | 32.10 | 22.30 | 10.50 | 1.60 | 0.985 |
L2-3 | 65.00 | 35.40 | 23.00 | 21.10 | 13.20 | 2.10 | 0.984 |
L2-4 | 67.50 | 47.70 | 39.00 | 25.90 | 12.30 | 2.10 | 0.959 |
L2-5 | 70.30 | 41.50 | 30.00 | 21.30 | 15.00 | 2.70 | 0.978 |
E2 | 66.00 | 55.90 | 42.10 | 23.80 | 12.20 | 2.50 | 0.950 |
E6 | 68.90 | 47.20 | 35.70 | 30.10 | 24.50 | 7.90 | 0.987 |
E7 | 66.70 | 55.90 | 48.10 | 26.70 | 25.20 | 5.80 | 0.958 |