Fig. 4
From: Bronchial biopsy specimen as a surrogate for DNA methylation analysis in inoperable lung cancer
Comparison of methylation levels of CpGs across different kinds of samples. a–c Methylation levels of three CpGs (cg07122178, cg01268824, and cg03826594) at SFRP1, ZNF154, and ITGA5 genes were compared in bronchial washing samples from 76 lung cancer patients (BW_cancer) and 60 healthy individuals (BW_control), bronchial biopsies from 8 lung cancer patients (LC_biopsy), 42 lung tumor (LC_tumor) and matched normal tissues (LC_normal), and in sputum from 12 lung cancer patients (LC_sputum). d–f Methylation levels of three CpGs (cg12600174, cg15377283, and cg17495130) at HOXA9, SOX17, and HOXD13 genes were compared using parallel coordinate plots between paired bronchial biopsy and bronchial washing specimens from eight lung cancer patients. Methylation levels were significantly higher in bronchial biopsy than in bronchial washing samples (P < 0.05, Wilcoxon signed-rank test). Y-axis indicates β-values from the 450K array