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Table 3 The mean methylation status of the five genomic regions in the validation datasets

From: Targeted bisulfite sequencing identified a panel of DNA methylation-based biomarkers for esophageal squamous cell carcinoma (ESCC)

Genomic regiona No. CpG sitesb CpG site included Gene McaMc McoMc P valued log10(OR)e 95% CIe Sens Spec AUC
chr8:99952469-99952722 19 cg15830431 STK3 0.35 0.16 4.20E−09 2.82 1.83–4.03 0.64 0.82 0.76
chr19:40314817-40314928 6 cg19396867 NA 0.36 0.12 9.60E−11 2.90 1.97–4.03 0.61 0.90 0.79
chr19:40314939-40315133 17 cg20655070 NA 0.31 0.12 1.80E−09 3.61 2.42–5.06 0.60 0.90 0.77
chr19:58446187-58446437 19 cg26671652 ZNF418 0.50 0.26 1.10E−13 3.46 2.52–4.54 0.74 0.86 0.84
chr19:56879517-56879735 25 cg27062795 ZNF542 0.41 0.14 5.20E−13 2.81 1.94–3.86 0.71 0.84 0.83
  1. The sensitivity, specificity as well as the AUC were both with a logistic regression prediction model without adjustment for gender, age and smoking status and alcohol status
  2. Sens sensitivity, Spec specificity, AUC area under the curve
  3. aGenomic region represents the genomic coverage of the reads with targeted bisulfite sequencing, and the genomic coordinates shown here is based on the hg19 version of the genome
  4. bNo. CpG sites represents the number of the CpG sites in each region
  5. cMcaM represents the mean methylation percentage of the cases in each region, which consists of several CpG sites, while the McoM represents the mean methylation percentage of the controls in each region
  6. d P value is calculated through the Wilcoxon rank-sum test following with FDR (false discovery rate) adjustment for multiple correction
  7. eOR and 95% CI were conducted through logistic regression