Fig. 2From: TFF1 hypermethylation and decreased expression in esophageal squamous cell carcinoma and histologically normal tumor surrounding esophageal cellsTFF1 protein expression in ESCC and healthy esophagus. Representative images of TFF1 immunostaining for each sample group are shown. a, b Lung adenocarcinoma, used as positive control (a, × 20 magnification, b × 40 magnification). c, d Healthy esophagus, cytoplasmatic expression of TFF1 was detected in submucosa glands of all samples evaluated (n = 22; C, × 20 magnification, d, × 10 magnification). e, f Normal-appearing surrounding mucosa from ESCC patients, all samples (n = 7) were negative for TFF1 staining (e and f × 20 magnification). g, h ESCC, all samples (n = 48) were negative for TFF1 staining (g and h, × 20 magnification)Back to article page