Lung function discordance in monozygotic twins and associated differences in blood DNA methylation

Clinical Epigenetics

Table 4 Overview of results from EWAS analyses for intra-pair difference in change in lung function during the follow-up period (p value < 10⁻⁵)

Lung function measure	Probe	Estimate	p value	Chromosome	Bp (hg19)	Proximal gene	CGI feature	Methylation^a of “inferior” twin
Log-ΔzFEV1-change_IP	cg19484381	− 0.055	1.55E−06	6	28,890,673	TRIM27	Body-shore	Hyper
Log-ΔzFEV1-change_IP	cg27261494	− 0.081	5.77E−06	6	74,104,097	DDX43	TSS200-shore	Hyper
Log-ΔzFVC-change_IP	cg12796186	− 0.066	3.28E−06	1	10,458,599	PGD	TSS1500-island	Hyper
Log-ΔzFVC-change_IP	cg14514174	− 0.044	6.39E−06	9	99,181,512	ZNF367	TSS1500-island	Hyper
ΔzFEV1/FVC-change_IP	cg00552805	0.0551	9.84E−06	7	44,119,858	POLM	Body-shore	Hypo
	cg06375580	0.0449	9.25E−06	12	42,538,820	GXYLT1	TSS200-island	Hypo
	cg12733656	0.0396	8.82E−06	7	6,388,695	C7orf70	TSS200-island	Hypo

Bold indicates significance p < 1 × 10⁻⁶. Probes were annotated with the most proximal gene, genomic position Bp (hg19), and CpG island context (CGI feature)
Estimate intra-pair difference in M value (logit-transformed beta), TSS200 region spanning from transcription start site (TSS) to 200 bp upstream of TSS, TSS1500 region spanning from − 200 to − 1500 bp upstream of TSS, 5′UTR 5′ untranslated region, IGR intergenic region, NA not applicable, hyper hyper-methylation, hypo hypo-methylation
^aRelative DNA methylation of “inferior” twin compared to “superior” twin

ISSN: 1868-7083