Fig. 9

Effects of PAE on BrdU incorporation and cell proliferation in frontal cortex and hypothalamus of rat brain. Representative immunofluorescence images, at 20X, visualized using the fluorescence microscope Nikon Eclipse 2000 (Nikon, Melville, NY) are shown. Higher magnification of BrdU signal is shown in insets. BrdU positive cells were counted in frontal cortex (a) and hypothalamus (b) of PF and AF animals using ImageJ (National Institutes of Health) and quantifications are shown in (c). Data are shown as mean ± SE, n = 4–6 animals per group done in duplicates, *p < 0.05, ***p < 0.001, AF vs PF