Fig. 2
From: Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure
Effects of PAE on the fractional synthesis of replication-dependent canonical histones and histone variants in frontal cortex and hypothalamus of developing rat brain. We measured the incorporation of 2H in the canonical histone peptides H2A (vTIAQGGVLPNIQAVLLPkkTESHHk), H2B (iLLPGELAkHAVSEGTkAVTkYTSAK), H3 (kQLATkAAR), and H4 (gkGGkGLGkGGAkR) and histone variant peptides H3.3 (kSAPSTGGVkkPHR), H2Az (aTIAGGGVIPHIHkSLIGkkGQQkTV), and H2Ax (kGHYAER) in frontal cortex (a, c) and hypothalamus (b, d) of 6-day-old rat pups following a 5-day alcohol feeding and administration of 2H2O. Histone Fractional Synthesis Rate (FSR) was calculated as described in the “Methods” section, and data are shown as mean ± SE, n = 5–8 per group, *p < 0.05, **p < 0.01, ***p < 0.001, AF vs PF. e Western-blot analysis of histone proteins in whole brain of PF and AF animals. The reference protein β-actin is also shown