Fig. 5

PCR array identifies metastasis and invasion downstream genes of miR-335. a, b Expression profiles of genes involved in metastasis and tumor invasion in AGS cells transfected with miR-335 mimic or with miR-335 inhibitor. Expression levels were normalized to three endogenous genes. Results indicate the mean values of three independent experiments ± SD. c ΔCq of PLAUR in 19 gastric tumor tissues compared to their matched non-tumor adjacent tissues (NAT) and normalized to reference genes. Data were transformed to logarithmic values (−log). d Relative expression of PLAUR in 19 samples of gastric tumor tissues, normalized to reference genes. e ΔCq of CDH11 in 19 gastric tumor tissues compared to NAT and normalized to reference genes. Data were transformed to logarithmic values (−log). f Relative expression of CHD11 in 19 samples of gastric tumor tissues, normalized to reference genes. Data were transformed to logarithmic values (log 2). g Direct interaction between miR-335 and CDH11 was detected by dual-luciferase reporter assay. Over-expression of miR-335 in AGS cells reduced the luciferase signal of CDH11 compared with NC mimic, while mutation of the miR-335-binding site diminished this suppressive effect. *р < 0.05, **p < 0.01