Fig. 3
From: MicroRNA-335-5p is a potential suppressor of metastasis and invasion in gastric cancer
Overexpression of miR-335 reduces clonogenic capacity, anchorage-independent cell growth and cell viability. a, b Representative images for AGS cells transfected with control NC/miR-335 mimic or with NC/miR-335 inhibitor in clonogenic assay. Crystal violet-stained colonies are shown. c AGS cells transfected with control NC/miR-335 mimic in soft agar assay. Formation of colonies > 50 μm in diameter was scored at 3 weeks. d, e Viability of AGS cells transfected with control NC/miR-335 mimic or with NC/miR-335 inhibitor in MTS assay. f Exogenous expression of miR-335 in AGS cells in trypan blue dye exclusion assay. Results represent the means of three independent experiments, bars indicate SD. *p < 0.05, **p < 0.01, ***p < 0.001. WT, wild-type