Table 3 Correlations between DNA methylation of metformin transporter genes in human liver and metabolic phenotypes including fasting glucose and BMI with P values < 0.05 in subjects from the Kuopio Obesity Surgery Study (n = 95)
Probe ID | Chr. | Position | Gene region | B (P value) | q value |
|---|---|---|---|---|---|
GLUCOSE (mmol/L)a | |||||
SLC22A1 | |||||
cg24864413 | 6 | 160542732 | TSS200 | 0.73 (0.001) | 0.013 |
cg22416916 | 6 | 160542770 | TSS200 | 0.52 (0.011) | 0.065 |
cg13466809 | 6 | 160542944 | 1stExon;5’UTR | 0.39 (0.027) | 0.070 |
cg27292431 | 6 | 160543261 | 1stExon | 0.61 (0.020) | 0.065 |
cg07558837 | 6 | 160555312 | Body | 0.22 (0.015) | 0.065 |
Average DNA methylation (13 CpG sites) | 0.33 (0.002) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (4 CpG sites) | 0.60 (0.007) | ||||
SLC22A3 | |||||
cg06295784 | 6 | 160771074 | Body | 0.62 (0.004) | 0.046 |
cg07883823 | 6 | 160769116 | TSS1500 | 0.30 (0.002) | 0.046 |
cg11696576 | 6 | 160815734 | Body | 0.57 (0.025) | 0.143 |
cg22117918 | 6 | 160769114 | TSS1500 | 0.16 (0.043) | 0.198 |
cg25313204 | 6 | 160768801 | TSS1500 | 0.65 (0.008) | 0.061 |
Average DNA methylation (23 CpG sites) | 0.14 (0.056) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (5 CpG sites) | 0.21 (0.008) | ||||
SLC47A1 | |||||
cg01530032 | 17 | 19435805 | TSS1500 | 0.91 (0.002) | 0.016 |
cg07829432 | 17 | 19437013 | TSS200 | 0.13 (0.001) | 0.012 |
cg08895056 | 17 | 19438253 | Body | 0.49 (0.031) | 0.136 |
cg11784214 | 17 | 19479935 | Body | 0.19 (0.047) | 0.161 |
cg12550399 | 17 | 19482275 | 3′ UTR | 1.14 (0.001) | 0.012 |
cg15014549 | 17 | 19437003 | TSS200 | 0.06 (0.034) | 0.136 |
cg24151087 | 17 | 19450271 | Body | 0.24 (0.020) | 0.120 |
Average DNA methylation (24 CpG sites) | 0.16 (0.005) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (11 CpG sites) | 0.20 (0.012) | ||||
BMI (kg/m2)b | |||||
SLC22A1 | |||||
cg13434757 | 6 | 160541976 | TSS1500 | 0.22 (0.045) | 0.230 |
cg13466809 | 6 | 160542944 | 1stExon;5′UTR | 0.23 (<0.0001) | <0.0001 |
Average DNA methylation (13 CpG sites) | 0.09 (0.013) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (4 CpG sites) | 0.16 (0.038) | ||||
SLC22A3 | |||||
cg06295784 | 6 | 160771074 | Body | 0.28 (<0.0001) | <0.0001 |
cg09226986 | 6 | 160852328 | Body | − 0.12 (0.020) | 0.153 |
cg25313204 | 6 | 160768801 | TSS1500 | 0.31 (<0.0001) | <0.0001 |
Average DNA methylation (23 CpG sites) | 0.03 (0.261) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (5 CpG sites) | 0.06 (0.030) | ||||
SLC47A1 | |||||
cg01530032 | 17 | 19435805 | TSS1500 | 0.22 (0.029) | 0.174 |
cg10718608 | 17 | 19438221 | Body | 0.20 (0.003) | 0.072 |
cg12133118 | 17 | 19436770 | TSS1500 | 0.17 (0.006) | 0.072 |
cg12550399 | 17 | 19482275 | 3′ UTR | 0.26 (0.024) | 0.174 |
Average DNA methylation (24 CpG sites) | 0.06 (0.001) | ||||
DNA methylation promoter region: TSS1500 and TSS200 (11 CpG sites) | 0.06 (0.025) | ||||