CpG location and methylation level are crucial factors for the early detection of oral squamous cell carcinoma in brushing samples using bisulfite sequencing of a 13-gene panel

Table 3 The best and worst AUC values among the 18 genes evaluated in this study. KIF1A showed a minimal variation among all 27 CpGs interrogated, whereas TERT displayed the widest gap between different AUC, highlighting the importance of CpG location

Gene	Best AUC position	Best AUC value	Worst AUC position	Worst AUC value	Best to worst AUC gap
KIF1A	Chr2: 241759621	0.94	Chr2: 241759702	0.87	0.0694444
MIR137	Chr1: 98046097	0.78	Chr1: 98046237	0.69	0.0916666
GP1BB	Chr22: 19710956	0.97	Chr22: 19710954	0.88	0.0921717
H19	Chr11: 2018169	0.66	Chr11: 2018228	0.56	0.0992425
LRRTM1	Chr2: 80531799	0.9	Chr2: 80531776	0.8	0.1012626
NTM	Chr11: 131781167	0.86	Chr11: 131781080	0.75	0.1063131
ZAP70	Chr2: 98340854	0.96	Chr2: 98340816	0.85	0.1073232
MIR193A	Chr17: 29886944	0.84	Chr17: 29886881	0.72	0.1252525
EPHX3	Chr19: 15342885	0.83	Chr19: 15342831	0.71	0.1260101
LINC00599	Chr8: 9760888	0.83	Chr8: 9760805	0.69	0.1436869
FLI1	Chr11: 128564158	0.84	Chr11: 128564065	0.67	0.1643940
PAX1	Chr20: 21686312	0.9	Chr20: 21686253	0.74	0.1648990
ITGA4	Chr2: 1823229.02	0.84	Chr2: 182323028	0.66	0.1813131
PARP15	Chr3: 122296586	0.84	Chr3: 122296671	0.65	0.1919191
TERT	Chr5: 1279758	0.92	Chr5: 1279838	0.67	0.2563132
MIR296	Chr20: 57392374	0.75	Chr20: 57392419	0.44	0.3151516
DNMT1	Chr19: 10305597	0.67	Chr19: 10305652	0.34	0.3338384
TERC	Chr3: 169482446	0.69	Chr3: 169482370	0.28	0.4047980

ISSN: 1868-7083