Fig. 1
The expression and methylation status of DIRAS1 in colorectal cancer cells. a Top panel: semi-quantitative RT-PCR shows DIRAS1 expression levels in colorectal cancer cell lines: DKO, DLD1, HCT116, HT29, LoVo, LS180, RKO, SW48, SW480, and SW620. DAC 5-aza-2′-deoxycytidine, GAPDH internal control of RT-PCR, H 2 O double distilled water. (-) absence of DAC. (+) presence of DAC. Bottom panel: MSP results of DIRAS1 in colorectal cancer cell lines. U unmethylated alleles, M methylated alleles, IVD in vitro methylated DNA, serves as methylation control, NL normal lymphocytes DNA, serves as unmethylation control. b BSSQ results of DIRAS1. MSP PCR product spanned 143 bp in DIRAS1 promoter region. Bisulfite sequencing region is located 284 bp upstream of transcription start site in the genome. TSS transcription start site