Fig. 4
From: Epigenetic assays for chemical biology and drug discovery
Time-resolved fluorescence resonance energy transfer histone deacetylase assay. A signal is generated when the deacetylated peptides are captured by the Europium-labelled antibody donor and streptavidin-ULightâ„¢-acceptor thus bringing the Europium-donor and ULightâ„¢-acceptor molecules into close proximity. Upon irradiation at 340Â nm, the energy from the Europium-donor is transferred to the ULightâ„¢-acceptor, which, in turn, generates a signal at 665Â nm