Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Table 5 Sex differences for LINE1 methylation pyrosequencing assay

Cohort	Group	Males		Females		P values^a
Cohort	Group	Number of placentas	Global methylation fraction (mean ± SD)	Number of placentas	Global methylation fraction (mean ± SD)	P values^a
Original cohort	Controls	33	0.5091 ± 0.0463	32	0.4902 ± 0.0448	0.0494
Original cohort	ART	56	0.4785 ± 0.0402	70	0.4791 ± 0.0503	0.4727
Validation cohort	Controls	29	0.4266 ± 0.0401	28	0.3936 ± 0.0583	0.0078
Validation cohort	ART	26	0.4227 ± 0.0382	29	0.4386 ± 0.09538	0.2149
Opposite sex twins	ART	39	0.4076 ± 0.0294	39	0.4098 ± 0.0320	0.3199

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