Table 1 Primers used in qPCR
Gene name | 5′->3′ primer | 3′->5′ primer |
|---|---|---|
DDX58 | CCAGCATTACTAGTCAGAAGGAA | CACAGTGCAATCTTGTCATCC |
Env-Fc2 | CTCCATTAGTAGCAGTTCCTCTCC | GAGAATAGTGGGACCTGTCCTTT |
IFI27 | ACCTCATCAGCAGTGACCAGT | ACATCATCTTGGCTGCTATGG |
IFI44 | GAGAGATGTGAGCCTGTGAGG | TTTTCCTTGTGCACAGTTGAT |
IFI44L | GTGGATGATTGCAGTGAGGTT | AATATCCTTCATGGGGTCCAG |
IFI6 | TGCTACCTGCTGCTCTTCAC | CGAGCTCTCCGAGCACTTTT |
IL28A | TCCAGTCACGGTCAGCA | CAGCCTCAGAGTGTTTCTTCT |
IL29 | GAAGACAGGAGAGCTGCAACGTGGA | GGTTCAAATCTCTGTCACCACA |
IRF7 | CTGAGGGCTTGTAG | TCAACACCTGTGACTTCATGT |
ISG15 | GCCTCAGCTCTGACACC | CGAACTCATCTTTGCCAGTACA |
MLT1C49 | TATTGCCGTACTGTGGGCTG | TGGAACAGAGCCCTTCCTTG |
MLT2B4 | GGAGAAGCTGATGGTGCAGA | ACCAACCTTCCCAAGCAAGA |
OASL | GCAGAAATTTCCAGGACCAC | CCCATCACGGTCACCATTG |
RPLP0 | CAGACAGACACTGGCAACA | ACATCTCCCCCTTCTCCTT |
Env-Fc2 methylated | CGGGCGAGGTTTATTGGTTCG | CGCYTATTCCCWTTTATTCCCG |
Env-Fc2 unmethylated | TGGGTGAGGTTTATTGGTTTGTAGG | CACYTATTCCCWCCATTTATTCCCA |
Env-Fc2 COBRA | GTATAATGGGGAAAAATGGAA | ACTTCCCTAACCCTCCC |