Fig. 2

Transient treatment with RRx-001 induced a sustained IFN response in HCT 116 cells. Expression of IFN-stimulated genes (ISGs) over the course of 4 weeks in response to 24 h of treatment with 0.5 μM 5-AZA (a) or RRx-001 (b). Transcript levels of ISGs (ISG15, IRF7, OASL, and DDX58) were determined by qPCR 7 days after treatment. Upregulation of gene expression observed in 5-AZA- treated cells was statistically significant at all time points. Gene expression changes observed in RRx-001-treated cells that were not significantly different from DMSO-treated cells were marked by # and those that were significantly downregulated by ##