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Table 1 Clinical and cytological characteristics of the study population

From: Molecular Pap smear: HPV genotype and DNA methylation of ADCY8, CDH8, and ZNF582 as an integrated biomarker for high-grade cervical cytology

Characteristics Number Percentageg
Clinical
 Agea
  Median (IQR) 28 (24–35)
  Range (year) 19–67  
 Race/ethnicitya
  Asian [NILM, LSIL, HSIL] 9 [5, 3, 1] (3)
  Black [NILM, LSIL, HSIL] 40 [14, 15, 11] (14)
  White [NILM, LSIL, HSIL] 124 [48, 40, 36] (45)
  Other [NILM, LSIL, HSIL] 65 [21, 28, 16] (24)
  Unknown [NILM, LSIL, HSIL] 39 [12, 14, 13] (14)
Cytologicalb
 Total LBC samples collected 400 (100)
  LBC samples excludedc 123 (31)
   NILM 33  
   LSIL 85  
   HSIL 5  
  LBC samples included 277 (69)
   NILM 100  
   LSIL 100  
   HSIL 77  
 Sourced
  Cervical 276 (99.6)
  Vaginal 1 (0.4)
 Diagnostic categoryd
  Normal 100 (36)
  Abnormal 177 (64)
 Cellular DNA concentrationd,e,f
  NILM 100  
   Median (ng/μL) (IQR) 48.1 (37.1–74.3)
   Range (ng/μL) 20.8–181.5  
  LSIL 100  
   Median (ng/μL) (IQR) 46.3 (35.6–63.1)
   Range (ng/μL) 20.5–182.8  
  HSIL 77  
   Median (ng/μL) (IQR) 51.8 (34.9–71.3)
   Range (ng/μL) 11.5–154.0  
  1. IQR interquartile range, LBC liquid-based cytology, LSIL low-grade squamous intraepithelial lesion, HSIL high-grade squamous intraepithelial lesion, NILM negative for intraepithelial lesion and malignancy
  2. aAge and race/ethnicity of subjects (N = 277) are based on the demographic data of included samples. The number of samples by race/ethnicity and cytological diagnosis are placed in square brackets. The distribution of the five categories of race/ethnicity (inclusive of Other and Unknown) among the three cytological grades were not significantly different (χ 2, p = 0.777)
  3. bCytopathology results are ascribed to the specimens collected on the day of the study enrollment
  4. cExclusion criteria included: low cell pellet volume (<200 μL); low cellular DNA concentration (<20 ng/μL); low nucleic acid purity (spectrophotometry absorbance ratio 260/230 nm <0.7); excess samples (>100) (see text for details)
  5. dData based on included samples (N = 277)
  6. eConcentration of total cellular DNA per sample after manual or semi-automated DNA extraction
  7. fComparison of DNA concentrations between NILM, LSIL, and HSIL samples were not significantly different (p = 0.519) by Kruskal-Wallis test
  8. gValues are N (%) unless otherwise denoted