Table 2 Descriptive and inference statistics of the two assays
From: Disagreement between two common biomarkers of global DNA methylation
Descriptives | Paired tests | Method of 95 % limits of agreement between measurements (“Bland-Altman”) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Data subset (cell type) | LINE-1 (mean ± SD and (range)) (%) | LUMA (mean ± SD and (range)) (%) | Wilcoxon signed rank test | Spearman Correlation (95 % CI) | Mean difference (fixed bias) (95 % CI) | Significance of mean deviation from 0 | KS-test of normal distribution of differences | 95 % confidence limits of agreement | Slope (proportional bias) (CI of estimate) | Significance of deviation of slope from 0 |
MCF7 | 60.96 ± 11.99 (34, 76.18) | 67.8 ± 11.99 (34.36, 78.34) | V = 2476, p = 6.22 × 10−10 | ρ = 0.58 (0.38, 0.74), p = 5.53 × 10−8 | −6.8 (−8.3, −5.3) | 1.93 × 10−13 | D = 0.1183, p = 0.24 | −19.5, 5.8 | −0.00046 (−0.13, 0.13) | 0.99 |
SHSY5Y | 59.01 ± 11.54 (31.91, 71.65) | 55.66 ± 11.99 (24.89, 67.65) | V = 74, p = 4.66 × 10−5 | ρ = 0.8 (0.56, 0.92), p = 1.31 × 10−8 | 3.3 (1.9, 4.8) | 3.16 × 10−5 | D = 0.068241, p = 0.99 | −4.6, 11.3 | −0.04 (−0.16, 0.08) | 0.53 |
Human blood | 92.4 ± 2.75 (77.9, 98.8) | 72.3 ± 3.1 (61.9, 78.5) | V = 0, p <2.2 × 10−16 | ρ = 0.35 (0.18, 0.51), p = 3.25 × 10−5 | 20.1 (19.5, 20.7) | 3.41 × 10−103 | D = 0.086144, p = 0.29 | 13.4, 26.8 | −0.2 (−0.44, 0.045) | 0.11 |