Fig. 9
JMJD6 increases the metastatic propensity of c-Myc-expressing cells. Transwell migration (a) and invasion (b) assays of 88CT1 cells overexpressing JMJD6 compared to LacZ control (*p < 0.05, **p < 0.01, respectively). c Western blot analysis of EMT markers in the cells used in a and b shows increased expression of Snail and Twist1 in cells with high expression of JMJD6. d Lung colonization in vivo. 5 × 105 cells used in a and b were injected by tail vein into FVB/N mice. After 20 days, the lungs were formalin fixed and stained with H&E. e Quantitation of metastatic nodules per lung of mice shown in d