Fig. 1

Bisulfite sequencing of exon1 of ADAMTS19 in 15 CRC cases. a Scheme of the 600 bp region surrounding the first exon of ADAMTS19. The transcriptional start site (TSS) and 5′ untranslated region (UTR) are indicated. In pink is an internal region of the ADAMTS19 CpG island (CGI), with vertical bars indicating every CpG site. The dashed blue area indicates the band C-19 initially detected by MS-AFLP. The region amplified for bisulfite sequencing is represented by the gray rectangle, with the BstUI sites indicated by vertical bars. For a larger view of this region, see Additional file 1: Figure S1. b On the left side are cases that were scored as unmethylated by MS-AFLP. On the right are cases that were scored as hypermethylated. Every horizontal line represents individual cloned sequences. Every circle represents 1 of the 34 CpG sites within the region studied. The scale is relative to the codon +1. In black and white, represented are the methylated and unmethylated CpG sites, respectively. Cases with asterisks are included in the representative results of the BstuI COBRA analysis (c). MWM molecular weight marker (100 bp ladder), N normal sample, T tumor sample, C+ positive control (human methylated DNA), C− negative control (human non-methylated DNA), U undigested, D digested. Methylation is indicated by the presence of smaller digestion products