Fig. 1

A gene expression profile links DNMT1 depletion with CG gene activation in melanoma cell lines. a Establishment of a CG gene activation score (CGAS) from the 45-MelCells dataset based on the expression profile of 11 selected CG genes (listed on the left). b Graphic representation of genes showing differential expression levels between the groups of melanoma cell lines displaying a CGAS either ≤2 or ≥7. The genes were classified from top to bottom according to their difference in mean expression. Two representative PCCG genes and four ICCG genes used in subsequent analyses are highlighted. c Functional analysis of PCCG and ICCG genes (via DAVID Bioinformatics Resources 6.7). Each bar in the histogram represents the Fisher exact p value (in a base 10 logarithmic scale) associated with the corresponding function in the group of genes. d Cross-comparison between the set of DNMT1-regulated genes (n = 346, see ref. [28]) and either the PCCG or ICCG group of genes. Overlap scores are expressed as ratio between observed and expected matches in a base 2 logarithmic (log2) scale. Statistical analysis was obtained by a Fisher test and compares in each group the number of observed genes vs the number of expected genes. ****p < 0.0001