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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Nucleated red blood cells impact DNA methylation and expression analyses of cord blood hematopoietic cells

Fig. 1

DNAm profiles of cord blood cells isolated by the standard FACS strategy. a A CD14-/CD19-/CD3+/CD235+ population isolated by FACS (left panel) is revealed to be T cell/RBC doublets by flow cytometry, which identifies two distinct cell types after sorting (right panel). b Unsupervised Euclidean clustering of genome-wide DNAm (440,315 CpG sites) between whole T cells (CD3T), nRBCs, and monocytes (Mo); numbers in the sample labels indicate different cord blood donors. c Number of large magnitude DM sites (FDR <5 %, |Δβ| >0.20) between nRBCs and T cells, nRBCs and monocytes, and T cells and monocytes sorted using a standard approach. d DNAm heatmap of nRBCs, T cells, and monocytes at top nRBC DM sites identified by the standard sorting protocol (FDR <5 %, |Δβ| >0.30; 457 CpG sites)

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