Fig. 1
From: 5-Carboxylcytosine levels are elevated in human breast cancers and gliomas
Both DAB-based and fluorescent immunohistochemistry protocols are suitable for 5caC detection in paraffin-embedded tissues. DAB-based detection of 5caC in 12.5 dpc mouse embryonic brain tissue (a) and adult mouse cortex (b). c-d Confocal image of 12.5 dpc mouse embryonic brain tissue immunostained for 5hmC and 5caC using tyramide-based fluorescent immunochemistry. The inset shows the magnified field used for the generation of 2.5XD signal intensity plots presented in (c). Merged views only are shown in (c) and the merged view together with individual channels in (e). Scale bar is 10 μm. e DAB-based detection of 5hmC in 12.5 dpc mouse embryonic brain tissue