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Table 1 Linearities, LOQs, and LODs for 5-mdC and 5-hmdC obtained by on-line trapping/cHILIC/ESI-MS

From: DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry

Analytes Linear range (vs.[dC],%) Regression line R 2 LOD (fmol) LOQ (fmol)
Slope Intercept
5-mdC 0.1–10 0.0193 ± 0.0007 −0.0017 ± 0.0003 0.9951 0.04 0.13
5-hmdC 0.002–0.1 0.0126 ± 0.0002 0.0001 ± 0.00001 0.9997 0.19 0.62