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Table 1 Linearities, LOQs, and LODs for 5-mdC and 5-hmdC obtained by on-line trapping/cHILIC/ESI-MS

From: DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry

Analytes

Linear range (vs.[dC],%)

Regression line

R 2

LOD (fmol)

LOQ (fmol)

Slope

Intercept

5-mdC

0.1–10

0.0193 ± 0.0007

−0.0017 ± 0.0003

0.9951

0.04

0.13

5-hmdC

0.002–0.1

0.0126 ± 0.0002

0.0001 ± 0.00001

0.9997

0.19

0.62

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Clinical Epigenetics

ISSN: 1868-7083

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