Fig. 8

Large genomic regions on chromosome 6 demonstrate correlations between new DNA methylation fold-enrichment and differential gene expression after A. phagocytophilum infection of human peripheral blood neutrophils. Chromosome 6 positions are displayed in Mb across the x-axis and the Spearman correlation coefficient (ρ) between fold-enrichment of newly meDNA and log₂ fold-change in differential gene expression (DGE) is plotted on the left y-axis. Light gray zones correspond to regions of chromosome 6 that are upregulated (positive) and downregulated (negative), black zones delineate the boundaries of the MHC locus, and the dark gray zone depicts the PROX region downstream of the MHC locus. Note that the x-axis is scaled to gene feature density and not linear position on the chromosome. The lower hatched line plot corresponds to the right y-axis and shows 1-(q value) for the Spearman correlation at the window centered at that genomic position such that peaks correspond to low q values. The dotted line demarcates where the 1-q value is above 0.90 which is equivalent to a q value <0.10. MeDNA data are from the average of three donors. Expression data are derived from GSE2405 [9]