Fig. 4
Infection of human neutrophils with A. phagocytophilum increases DNA methylation upstream of promoters and far downstream of transcription termination sites. New regions of DNA methylation enrichment were investigated using CEAS to determine the percentage that were present in a promoters, b downstream of genes, and c in bi-directional promoters. Only the most significant peaks, those with a −10*log10 (p value) > 100, were analyzed. White bars denote the percentage of the genome annotated to that particular feature (genomic background), gray bars denote average percentage of regions of DNA methylation unique to A. phagocytophilum-infected human peripheral blood neutrophils (PMN.Aph), and black bars denote the average percentage of regions of methylation unique to uninfected cells (PMN); error bars are standard error of the mean (sem). Average values are calculated using all three donors. Tables in d show the exact percentage of DNA methylation enrichment for each particular feature where PMN.Aph represents regions unique to infected cells, PMN represents regions unique to uninfected cells, and sem for each group