Fig. 1

Anaplasma phagocytophilum induces genome-wide changes in DNA methylation of human peripheral blood neutrophils. The quantity of new regions of DNA methylation was compared between infected and uninfected samples. a Pie charts demonstrate the proportion of new methylation peaks among all meDNA that mapped to infected cells only (left dark wedge) versus uninfected cells (right dark narrow wedge). While a large percentage of peaks are shared between samples, infected samples have more new regions of methylation as shown by the Venn diagram. The number of methylated regions shown is the average of the three donors. b CEAS was used to linearly map chromosomal locations of all methylated peaks unique to infected or uninfected samples. Representative image of a single donor where regions of DNA methylation (bars) unique to A. phagocytophilum-infected neutrophils and to uninfected human neutrophils are shown per chromosome, by their linear location on the x-axis. Each bar represents a new region of methylation found only in its respective sample. Roughly 30,000 new regions of methylation are unique to infected samples and cover the entire genome. In comparison, the uninfected samples show an equally diverse distribution of ~1000 newly methylated regions