Fig. 3
From: MethPed: a DNA methylation classifier tool for the identification of pediatric brain tumor subtypes
Immunohistochemical analyses of two patients with challenging diagnoses. a Infant (case BPC C1) diagnosed according to the WHO criteria with a large-cell medulloblastoma, located in the vermis. H&E shows predominantly large cells with a high frequency of apoptotic bodies, clonal positivity of GFAP, and low positivity for synaptophysin and clonal areas with high Ki-67 positivity (objective original magnification × 40). b Four-year-old child (BPC B7) diagnosed with an intra- and periventricular PNET tumor. H&E shows high frequency of necrosis and vessels, very strong, clonal positivity of GFAP in tumor cells as well as positivity in reactive gliosis, high positivity for synaptophysin, and high Ki-67 positivity (objective original magnification ×40)