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Fig. 5 | Clinical Epigenetics

Fig. 5

From: DNA methylation levels are highly correlated between pooled samples and averaged values when analysed using the Infinium HumanMethylation450 BeadChip array

Fig. 5

Protocol for performing DNA Pools. First, DNA concentrations of individual samples were measured three times by ultraviolet (UV) light absorption (Nanodrop spectrophotometer). When readings differ less than 4 ng/μl, samples were diluted to 40 ng/μl. Next, the pools were prepared using 5ul of DNA from each of the 5 samples that would form part of the pool. The final concentration of every pool should be 25 μl at 40 ng/μl, with DNA concentration checked by UV light absorption (Nanodrop spectrophotometer) again. Finally, if readings were 40 ng/μl ±4 ng/μl, pools were analysed with the EWAS arrays. If readings differed, the pools were created again from the first step

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Clinical Epigenetics

ISSN: 1868-7083

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