Inflammation triggers specific microRNA profiles in human adipocytes and macrophages and in their supernatants

Clinical Epigenetics

Table 2 Testing of inflammation-induced miRNA candidates identified in vitro in human subcutaneous adipose tissue (AT) samples

	Inflamed (5% MCM)/control adipocytes				Inflamed (10 ng/ml LPS)/control macrophages				Obese AT/post-weight loss
	Expression		Supernatant		Expression		Supernatant		Human AT ( n = 9)
	Ratio	P value	Ratio	P value	Ratio	P value	Ratio	P value	% change	P value
miR-146b	Only detectable in the SN of inflamed MAs								−88	<0.0001
miR-146a					4.96	<0.0001			−69.6	0.002
miR-376c	Only detectable in inflamed MAs								−78	0.002
miR-221	1.98	0.027	15.43	0.002	0.49	0.034			−51.3	0.003
miR-223			4.87	0.001					−74.3	0.004
miR-411	Only detectable in inflamed MAs								−62.2	0.004
miR-155	5.01	0.015	4.03	<0.0001	2.48	0.001			−54.4	0.005
miR-19b			19.76	<0.0001	0.61	0.009			−72.7	0.006
miR-19a	Only detectable in the SN of inflamed MAs				0.49	0.032	2.02	0.034	−73.4	0.008
RNU48							0.22	0.015	−45	0.009
miR-222	2.51	0.044	6.07	0.001					−48.9	0.028
miR-193b			0.37	0.014					−44.8	0.039
miR-766	Undetectable in inflamed MAs								182	0.084
let-7a							0.12	0.014	6.1	0.276
miR-145					0.19	0.005	0.14	0.032	−26.9	0.228
miR-92a			7.73	0.002			1.75	0.007	−21.3	0.327
miR-572	Undetectable in inflamed MAs								−7.6	0.413
miR-378							4.95	0.014	−41	0.78

Results from nine morbid obese participants before and after surgery-induced weight loss (post-weight loss, see Additional file 2: Table S2 for additional details). Results assessed were compared by paired t-test. ‘Detectable’ refers to <35 Cts, while ‘undetectable’ indicates ≥35 Cts in all biological replicates (n = 3), as explained above. Italics for significant results.

ISSN: 1868-7083