Summary of the analysis of the results of the peptide methylation experiments. Panels (A) and (B) show the average rate constants of MLL3-SET wild-type and MLL3-SET Y4884C variant for the methylation of H3K4me0 (A) and H3K4me1 (B) substrates determined at a peptide concentration of 10 μM. Error bars indicate the standard error of mean of two independent experiments. Data in panel (A) were normalized to k
1 of MLL3-SET, and data in panel (B) were normalized to k
2 of Y4884C. Panel (C) shows the v
values for methylation of the un- (me0), mono- (me1), or dimethylated (me2) peptide substrates by MLL3-SET and Y4884C. These values were determined from methylation kinetics carried using 5, 10, 20, and 40 μM of peptide (Additional file 1: Figure S5).