Figure 5
From: Somatic cancer mutations in the MLL3-SET domain alter the catalytic properties of the enzyme
Summary of the analysis of the results of the peptide methylation experiments. Panels (A) and (B) show the average rate constants of MLL3-SET wild-type and MLL3-SET Y4884C variant for the methylation of H3K4me0 (A) and H3K4me1 (B) substrates determined at a peptide concentration of 10 μM. Error bars indicate the standard error of mean of two independent experiments. Data in panel (A) were normalized to k 1 of MLL3-SET, and data in panel (B) were normalized to k 2 of Y4884C. Panel (C) shows the v max/K M values for methylation of the un- (me0), mono- (me1), or dimethylated (me2) peptide substrates by MLL3-SET and Y4884C. These values were determined from methylation kinetics carried using 5, 10, 20, and 40 μM of peptide (Additional file 1: Figure S5).