Table 2 The primer sequences used for high-resolution melting real-time PCR analysis
Primers | Sequence (5′-3′) | PCR product size (bp) | CpG sites ( n ) |
|---|---|---|---|
IL-4 F | AGGTTAGGAGATGGAGATTATTTTG | 102 | 4 |
IL-4 R | TAAAACTACAAACACCTACCACCAC | ||
IL-5 F | AGGTTAGGAGATGGAGATTATTTTG | 102 | 5 |
IL-5 R | TAAAACTACAAACACCTACCACCAC | ||
IFN-γ F | GAGTTTTGTTTTGTTATTTAGGTTGG | 124 | 6 |
IFN-γ R | AATACCTATAATCCCAACTACTC | ||
IL-10 F | TTTATTGTGTTAATTAGGATGGTTT | 154 | 7 |
IL-10 R | TAAACAAAAATCCAAAATACTTTTT |