The regulation of miR-1246 expression by AKT-P53 signaling pathway in B cells from systemic lupus erythematosus (SLE) patients. (A, B) AKT phosphorylation was markedly increased, but no difference in AKT protein expression (A, C), while P53 protein level was sharply downregulated (B, D) in B cells from active SLE patients compared to healthy controls. AKT phosphorylation levels were negatively correlated with P53 protein expression levels (E) while P53 protein levels were positively correlated with miR-1246 expression levels in B cells from active SLE patients (F). The Western blot images are representative images (n = 4). Bars in (C) and (D) show the mean ± SD results in 20 healthy donors and 30 active patients with SLE. (*P < 0.05).