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Table 4 Primers designed for pyrosequencing

From: Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Gene

Primers

Sequence to analyze

PCR product size (bp)

PRM_1

F

5′-GGG GTG GTT AGG GAT ATG T-3′

ATTTYGTGYGAGYGTTYGTTTAGGTTTTTTAYGYGGTATYGGATTATGGTGTTGGGAGATTTGGTGG

335

R

Bio5′-CAC CAA ATC TCC CAA CAC CAT-3′

S

5′-ATG TAA TTG TTG TTT GTA T-3′

PRM_2

F

Bio5′-GGG GTG GTT AGG GAT ATG T-3′

ACCRAATCCACAAACRACAACATCRCTCCTACAAAAAACRCAAAAAACRCTCCTACAAACACC

182

R

5′-AAA AAC CCA TAA CCA ATC TCA CTA TAA-3′

S

5′-CAC TAC TCT CCA AAA AAA CTA C-3′

AFF4

F

5′-GGG TTA GGG TTG GGA TAG TTG A-3′

TTYGTTTYGTTYGTTGGYGGYGGYGAYGGTAGTTGGATTTTTGTAGTTAGGGT

229

R

Bio5′-AAA CCC CCC CCC CCT ACT-3′

S

5′-GAA GAT TTG GTA TTA GGA TT-3′

H19_4

F

5′-GGGGGTTTTTGTATAGTATATGGGT-3′

AATYGGAAGTGGTYGYGYGGYGGTAGTGTAGGTTTATATATTATAGTT

242

R

Bio5′-ACTCCTATAAATATCCTATTCCCAAAT-3′

S

5′-GGAATTGGTTGTAGTTGTGG-3′

NSD1m1

F

5′-AGTTGAGGATTTAGTAGGTTTGTATTTAG-3′

GTGTAGTYGTTTYGGTYGGTTYGTTTGYGGTTTGYGTATYGTYGTTGTAAAGGTTT

173

R

Bio5′-TACCCCCCCCTTCCTAACT-3′

S

5′-GGAAGGAGAGTTTTGGG-3′

  1. Table shows sequence to analyze and size of the PCR amplicons. F: forward primer; R: reverse primer; S: sequencing primer; Bio: biotinylation.