Skip to main content

Table 4 Primers designed for pyrosequencing

From: Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Gene Primers Sequence to analyze PCR product size (bp)
PRM_1 F 5′-GGG GTG GTT AGG GAT ATG T-3′ ATTTYGTGYGAGYGTTYGTTTAGGTTTTTTAYGYGGTATYGGATTATGGTGTTGGGAGATTTGGTGG 335
R Bio5′-CAC CAA ATC TCC CAA CAC CAT-3′
S 5′-ATG TAA TTG TTG TTT GTA T-3′
PRM_2 F Bio5′-GGG GTG GTT AGG GAT ATG T-3′ ACCRAATCCACAAACRACAACATCRCTCCTACAAAAAACRCAAAAAACRCTCCTACAAACACC 182
R 5′-AAA AAC CCA TAA CCA ATC TCA CTA TAA-3′
S 5′-CAC TAC TCT CCA AAA AAA CTA C-3′
AFF4 F 5′-GGG TTA GGG TTG GGA TAG TTG A-3′ TTYGTTTYGTTYGTTGGYGGYGGYGAYGGTAGTTGGATTTTTGTAGTTAGGGT 229
R Bio5′-AAA CCC CCC CCC CCT ACT-3′
S 5′-GAA GAT TTG GTA TTA GGA TT-3′
H19_4 F 5′-GGGGGTTTTTGTATAGTATATGGGT-3′ AATYGGAAGTGGTYGYGYGGYGGTAGTGTAGGTTTATATATTATAGTT 242
R Bio5′-ACTCCTATAAATATCCTATTCCCAAAT-3′
S 5′-GGAATTGGTTGTAGTTGTGG-3′
NSD1m1 F 5′-AGTTGAGGATTTAGTAGGTTTGTATTTAG-3′ GTGTAGTYGTTTYGGTYGGTTYGTTTGYGGTTTGYGTATYGTYGTTGTAAAGGTTT 173
R Bio5′-TACCCCCCCCTTCCTAACT-3′
S 5′-GGAAGGAGAGTTTTGGG-3′
  1. Table shows sequence to analyze and size of the PCR amplicons. F: forward primer; R: reverse primer; S: sequencing primer; Bio: biotinylation.