Figure 4

Gene expression changes upon long-term culture. Gene expression profiles (RNA-seq) were analyzed in MSCs of early passage (P4) and late passage (P13; n = 3). The volcano plot demonstrates differential expression upon long-term culture. Relevant genes are indicated in red (A). Particularly, genes localized at the border of LADs were hardly expressed (B). No significant association of senescence-associated gene expression changes was observed at the border of LADs (C). Differentially expressed genes are in genomic regions that are not localized in LADs (D). Gene ontology (GO) analysis was performed for genes, which were either significantly up- or downregulated compared to all genes (Fisher’s Exact test followed by Benjamin Hochberg multiple test correction). Shown are the most significant categories (E).