Figure 2

Senescence-associated DNAm changes. Scatter plots of global DNAm profiles (analyzed by methyl-capture sequencing) of early versus late passage are depicted for fibroblasts of donor 1 (A) and 2 (B) (DMR = differentially methylated region; log2 signal intensities are depicted for each DMR). Prominent DMRs were observed within the HOXC locus (C). Senescence-associated DNAm changes in fibroblasts (MethylCap-seq data) were then compared to DNAm changes upon long-term culture of MSCs (450k IlluminaBeadChip) of our previous work [14]. Differential DNAm levels (late minus early passage) in fibroblast 1 (D) and 2 (E) were analyzed at genomic regions surrounding the CpGs with senescence-associated DNAm changes in MSCs (450k IlluminaBeadChip data is plotted). This comparison reflected the overlap of senescence-associated DNAm changes between the two methods and cell types. Subsequently, we analyzed the mean DNAm level of neighboring CpGs in the 450k IlluminaBeadChip data of MSCs. We used a 500-bp window in the vicinity of CpGs with significant SA-hypermethylation (F) and SA-hypomethylation (G).