Effect of vitamin B
in the presence of methylation inhibitor. (A) Total cholesterol in cell lysate. (B) Homocysteine in conditioned media. Relative mRNA expression of (C) SREBF1, (D) LDLR, and (E) HMGCR were measured in adipocytes maintained in customised media control, NoB, and control + 5-Aza-dC. 5-Aza-dC is methylation inhibitor. All experiments were performed as triplicates. Values are mean ± SEM. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, P value compared to control.