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Table 2 NOMe-seq primer sequences and amplification conditions

From: Nucleosome positioning is unaltered at MLH1 splice site mutations in cells derived from Lynch syndrome patients

Region

NOMe-seq primers

Annealing (°C)

c.588 + 1G > T

F:TTGATATTTAGTGTGTGTTTTTGGYAAT

F/R = 54°C

R:CACATAATATCTTAAAAAATTCCAAAATAATA

F/RN = 56°C

RN:ATACCRACTAACARCATTTCCAAAAATAA

c.589-2A > G

F:TTAGGTATTTAGTATATAATGYAGG

F/R = 51°C

R:CACTATAAATATTTTCAAAACTAAAACCTTA

F/RN = 52°C

RN:CACAAAATCTAAAAAATTACATACACCTAA

 

c.790 + 1G > T

F:TAGGYATAGGAGGATTTTAAATGGATTAAGTT

F/R = 52°C

R:CAATTTCTTTAATAACAATRCCTATACCTAAA

F/RN = 52°C

RN:TTACTCRTAAAAACTCTAACACCATCAA

c.791-1G > C

F:GATGTGATGTGYATATTATTATAGAAATGTT

F/R = 55°C

R:TATCARCACCTCCTAATAAAATGAARCATA

F/RN = 56°C

RN:ATCCTTTTRCCAATAATATATAAAATTCACTCTA

c.884G > A

F:GGATGTGATGTGYATATTATTATAGAAATGTT

F/R = 58°C

R:CTATTATARCTTCCCAACTAACCCCARCAA

F/RN = 58°C

RN:CTACAARCTATCARCACCTCCTAATAA

c.1559-2A > T

F:TATTAGGAGGYTTAATTTAGGYTTTTTTGYTTAT

F/R = 58°C

R:ACCCTCACCACCTAATTCACAACATTTATAA

F/RN = 57°C

RN:ACTAARCAACTACCAAAAACTAARCTTCTTA

HSPA5

F: GAGAAGAAAAAGTTTAGATTTTATA

F/R = 56°C

R: AAACACCCCAATAAATCAATC