Figure 7

Colony assay of AML-3 and HL-60 leukemic cells after sequential treatment with 5-AZA-CdR (A), GSK-126 (G) or DZNep (D), and/or TSA (T) or MS-275 (M). The cells were treated with 20 nM 5-AZA-CdR and, at 24 h, the following drugs were added: (A) 1 μM GSK-126 and/or 20 nM TSA; (B) 500 nM DZNep and/or 200 nM MS-275; (C) 1 μM GSK-126 and/or 500 nM MS-275; (D) 5 μM GSK-126 and/or 1,000 nM MS-275. At 48 h the cells were counted and placed in soft agar for colony assay to determine cell survival. The results are expressed as mean ± SEM, n = 3. Statistical analysis for reduction in survival: (A) A + G + T > (T + G) P A<0.05; (B) A + D + M > (M + D) P A<0.05; (C) A + G + M > (A + G, M + G) P A<0.05; (D) A + G + M > (A + G, A + M, M + G) P A<0.05 (one way ANOVA).