Figure 2

Induction of apoptosis of leukemic cells after sequential treatment with 5-AZA-CdR (A), DZNep (D), and/or TSA (T). AML-3 cells (A) and HL-60 cells (B) were treated with 20 nM 5-AZA-CdR and, at 24 h, 500 nM (AML-3) or 1,000 nM (HL-60) DZNep and/or 40 nM (AML-3) or 80 nM (HL-60) nM TSA were added to the medium. At 48 h the drugs were removed and at 72 h the cells were analyzed for induction of apoptosis using Annexin V staining. The results are expressed as mean ± SEM, n = 3. Statistical analysis for induction of apoptosis: AML-3 and HL-60 cells: A + D + T > (A + D, A + T, T + D) P A<0.05 (one way ANOVA).