Figure 2

Clustering dendrograms of Beckwith-Wiedemann syndrome (BWS) patients and controls (samples were assigned arbitrary numbers) according to methylation state of (A) all imprinted genes (excluding H19 and KvDMR1) and (B) all non-imprinted genes. *BWS indicates samples from patients with BWS and C indicates control samples. Suffix (ART) denotes BWS patients conceived with ART. (A) Unsupervised analysis of cluster 1 versus cluster 2 showed significant grouping of controls versus BWS patients (P = 0.0054). No significant clustering was detected of (i) BWS patients conceived via ART compared to BWS patients conceived naturally (P = 0.3082) and (ii) controls versus BWS between cluster 3 and 4 (P = 0.52). (B) Analysis of methylation status of CpGs associated with non-imprinted genes showed significant clustering of controls versus BWS (P = 0.0402) but no significant clustering of BWS conceived by ART compared to BWS patients conceived naturally (P = 0.3308).